Expression data from FITC challenged skin of CLEC4C-DTR mice vs. littermate controls treated with diptheria toxin

CLEC4C-DTR exhibit exacerbated contact hypersensitivty to the FITC hapten. Here we assess the transcriptional differences in whole skin of FITC challenged CLEC4C-DTR+ mice and DTR- mice treated with diptheria toxin. Overall, CLEC4C-DTR upregualated 439 genes and downregulated 152 genes compared to transgene negative controls, filtering output on 2 fold expression level difference and FDR<0.05. Six samples were analyzed, one mouse was used per replicate and each replicate contained 1 FITC treated ear. Three replicates per group were amplified and hybridized to the Affymetrix Mouse Gene 1.0 ST array. Raw data was processed by Robust Multi-Array (RMA) method. Volcano plots and differential expression analysis was performed using Multiplot studio and Morpheus with a threshold of 2 fold change and a minimum P value significance threshold of 0.05 (after False Discovery Rate [FDR] correction for the number of tests).