Tuberculosis patient characteristics according to genotype for the more frequently observed Mycobacterium tuberculosis strain genotypes.

1 The Allopatric and Sympatric classification of M. tuberculosis strains was obtained from comparison of Portuguese spoligotypes with the fourth international spoligotyping database SpolDB4 [23]. This database classifies strains according to their ‘‘Shared international type” (SIT). Allopatric strains include strains with SITs 42, 150, 17, 53, 50, 1, 34, 47 (n = 191) and Sympatric strains include those strains with SITs 20, 64, 389, 244, 1106 (n = 148).2 The MIRU-VNTR plus web application (http://www.miru-vntrplus.org) was used to analyse spoligotype data of the M. tuberculosis isolates [39–40]. For minimum spanning tree analysis the Kruskal’s algorithm and force-directed graph layout for visualization according to the SIT using one single locus variation from the Node, SLV(1) was used to generate groupings into clonal clusters (CC).3M. tuberculosis lineages were classified using spoligotype data. Spoligotype designation was obtained from the SpolDB4 database (http://www.pasteur-guadeloupe.fr:8081/SITVITdemo) [14–15] and using the Spotclust program (http://www.rpi.edu/$bennek/EpiResearch) [38]. Lineage designation, East-Asian and Euro-American, was on Long Sequence Polymorphism (LSP) analysis [7,9].4 Drug susceptibility data was available for 86.7% (294/339) of the cases and was used to determine the percentage of multidrugresistant tuberculosis (MDRTB) cases to strain genotype.5 HIV = human immunodeficiency virus infection6 Age in years was expressed as the mean ± sd, sd = standard deviation.Tuberculosis patient characteristics according to genotype for the more frequently observed Mycobacterium tuberculosis strain genotypes.