Wide-field imaging of cortical response to sensory stimulation in GCaMP6f mice at different brain states

How the mouse cortex integrates sensory information during sleep is still unknown. To investigate the cortical responsiveness to external stimuli, we recorded activity over both cortical hemispheres in mice at different isoflurane-anesthetized brain states using wide field optical imaging with the fluorescent calcium indicator GCaMP6f. We compared the cortical activity dynamics following whisker stimulation in two conditions: deep and medium anesthesia in the same chronically implanted mice. Combining level-dependent modulation of slow wave, optical imaging and whisker stimulation, it was possible to perform large-scale spatial correlation between cortical regions during a response evoked by sensory stimulation. Moreover, as recordings are saved as tiff files, it is easy to work on them using Matlab or ImageJ programs. Our preliminary results show that changes in evoked complexity response may be related to changes in the anesthesia level.

搜集汇总

背景与挑战

背景概述

该数据集通过宽场钙成像技术，记录了表达GCaMP6f的小鼠在不同麻醉深度下对胡须刺激的皮层反应，揭示了脑状态对感觉处理的影响，数据以TIFF格式提供，易于分析。