Data computational model parameters of nociceptive medullary dorsal horn projection neuron from: Opposite regulation of medullary pain-related projection neuron excitability in acute and chronic pain

Pain hypersensitivity is associated with increased activity of peripheral and central neurons along the pain neuroaxis. We show that at the peak of acute inflammatory pain, superficial medullary dorsal horn projection neurons (PNs) that relay nociceptive information to the parabrachial nucleus reduce their intrinsic excitability and, consequently, action potential firing. When pain resolves, the excitability of these neurons returns to baseline. Using electrophysiological and computational approaches, we found that an increase in potassium A-current (IA) underlies the decrease in the excitability of medullary dorsal horn PNs in acute pain conditions. In chronic pain conditions, no changes of IA were observed, and medullary dorsal horn PNs exhibit increased intrinsic excitability and firing. Our results reveal an opposite modulation of the excitability of medullary dorsal horn projection neurons in acute and chronic pain conditions, suggesting a regulatory mechanism that, in acute pain c..., Methods
Animals 
Adult (9-13 weeks) C57BL/6J mice were used in all experiments unless stated otherwise. To specifically express Channelrhodopsin 2 (ChR2) in transient receptor potential vanilloid receptor 1 (TRPV1) expressing neurons, we used homogeneous TRPV1cre mice (48) that were purchased from the Jackson Laboratory (#017769) and bred them in-house. Animals were group-housed under a controlled temperature (21 ± 2°C) and humidity (55 ± 10%) on a 12h-12h light/dark cycle, with ad libitum access to food and water. All experimental procedures were approved by the Institutional Animal Care and Use Committees at the Hebrew University of Jerusalem (study approval number MD-21-16152).
 
Pain models
Acute pain model: UV-photokeratitis model
Corneal photokeratitis was induced as previously described (28, 29). Briefly, mice were anesthetized with a combination of 70 mg/kg ketamine and 0.8 mg/kg medetomidine, intraperitoneally (i.p.). Mice were placed on a heating pad, and one eye was..., , # Source data for main and supplementary figures and computational model parameters of nociceptive medullary dorsal horn projection neuron from: Opposite regulation of medullary pain-related projection neuron excitability in acute and chronic pain

## Description of the data and file structure

**For the Source DATA Dataset**

**Title of Dataset:**\
Electrophysiological and Behavioral Source Data for the Article: Opposite Regulation of Medullary Pain-Related Projection Neuron Excitability in Acute and Chronic Pain

**Dryad DOI:**\
[https://doi.org/10.5061/dryad.sqv9s4ncp](https://doi.org/10.5061/dryad.sqv9s4ncp)

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**Summary**

This dataset contains the raw data underlying the figures in the manuscript, derived from experiments examining the biophysical regulation of medullary dorsal horn projection neurons in murine models of acute and chronic pain. The measurements include intrinsic electrophysiological properties, potassium A-type current characteristics, and behavioral indicators...,