Histological analyses data for: Multinucleated giant cells are hallmarks of ovarian aging with unique immune and degradation-associated molecular signatures

The ovary is one of the first organs to exhibit signs of aging, characterized by reduced tissue function, chronic inflammation, and fibrosis. Multinucleated giant cells (MNGCs), formed by macrophage fusion, typically occur in chronic immune pathologies, including infectious and non-infectious granulomas and the foreign body response, but are also observed in the aging ovary. The function and consequence of ovarian MNGCs remain unknown as their biological activity is highly context-dependent, and their large size has limited their isolation and analysis through technologies such as single-cell RNA sequencing. In this study, we define ovarian MNGCs through a deep analysis of their presence across age and species using advanced imaging technologies as well as their unique transcriptome using laser capture microdissection. MNGCs form complex interconnected networks that increase with age in both mouse and nonhuman primate ovaries. MNGCs are characterized by high Gpnmb expression, a putative..., , , # Multinucleated giant cells are hallmarks of ovarian aging with unique immune and degradation-associated molecular signatures

[https://doi.org/10.5061/dryad.kh18932j4](https://doi.org/10.5061/dryad.kh18932j4)

## Description of the data and file structure

Histological quantification of MNGCs and postitive antibody staining was performed on tissue sections from mouse and non-human primate ovaries using ImageJ. To quantify the amount of ovarian area taken up by MNGCs (figure 1), the Trainable Weka Segmentation plugin in Fiji (NIH) was used to train the model on regions of non-ovary, non-MNGC ovary, and MNGCs. For figure 4, percent positive CD4 or CD3ε area was determined as DAB positive area over the total area of an ROI that included area of MNGCs, corpora lutea, or stroma.

### Files and variables

#### File: Paper\_raw\_data.xlsx

**Description:** Raw data from ImageJ analysis of Figures 1C, 1D, 4F, 4G, 4H.

##### Variables

* Figure 1C and 1D
* Age in months (mouse) and age in y...,