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ChIP-Seq of constitutively expressed Ndt80-Myc in K. lactis. Kluyveromyces lactis

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https://www.ncbi.nlm.nih.gov/bioproject/PRJNA355301
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The purpose of this experiment was to identify the genes bound by Ndt80 in K. lactis when constitutively expressed. Ndt80 was tagged with c-myc, the native Ndt80 promoter was replaced with the K. lactis Gal1 promoter and the protein was immunoprecipitated with a c-myc antibody. Cells were grown overnight in SRaffinose until log-phase, then in SRaffinose + 1.7% Galactose for 5 hours. Each experiment was repeated twice and sequenced on an Illumina HiSeq 4000. Overall design: K.lactis Ndt80-myc vs. untagged control, with two technical replicates for each
创建时间:
2016-11-29
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