Raw data associated with the article Piper, C. and Green, J.B.A. "Cap-to-bell stage molar tooth morphogenesis occurs through proliferation-independent sulcus sharpening and condensation-associated tension in the dental papilla", Journal of Anatomy 2025

This archive contains the raw data associated with the paper "Cap-to-bell stage molar tooth morphogenesis occurs through proliferation-independent sulcus sharpening and condensation-associated tension in the dental papilla". It shows, using mouse molar explant inhibition and cut-and-recoil experiments, that cap-to-bell stage morphogenesis is largely proliferation-independent (sulcus sharpening entirely so) (Fig.1) and that tension in the mesenchyme of the dental papilla, immediately sub-adjacent to the cusps, rather than compression by the mesenchyme surrounding the whole structure, is what holds the structure in shape (Figs. 2 and 3). Fine mapping of the degree of condensation (Fig.4) shows that it is highest in the mesenchyme of the dental papilla and becomes progressively more focused to the cusp regions, consistent with a key role in cusp shaping. Together these findings overturn the prevailing models of molar morphogenesis, including both cusp and sulcus formation. Files in this archive consist of raw (.tif) versions of images in the figures, similar images that were used to generate the graphs in the figures, morphometric (.morphoj) files containing all the measurements and process used to generate some of the graphs in Fig.1, R-code used to generate the cell packing/density heatmaps, and the StarDist machine-learning model used to segment the images of nucleus (DAPI) stained molar tooth slices to generate the nuclear location data (point cloud) used as input to the R-code.

本档案囊括了与论文《牙乳头中牙尖发育的帽状至钟状阶段形态发生主要依赖于增殖独立性的沟槽锐化与凝结相关张力》相关的原始数据。该研究通过小鼠磨牙切片抑制和切割-回缩实验，揭示了帽状至钟状阶段形态发生主要不依赖于增殖（沟槽锐化完全如此）（图1）以及牙乳头中髓质的张力，而非整个结构周围髓质的压缩，是维持结构形态的关键（图2和图3）。对凝结程度进行精细映射（图4）表明，凝结程度在牙乳头中髓质最高，并逐渐集中于牙尖区域，这与牙尖塑造的关键作用相一致。这些发现颠覆了现有的磨牙形态发生模型，包括牙尖和沟槽的形成。本档案中的文件包括图中图像的原始（.tif）版本、用于生成图中图表的类似图像、包含所有测量和生成图1中一些图表所使用过程的形态计量（.morphoj）文件、用于生成细胞堆积/密度热图的R代码，以及用于分割被DAPI染色的磨牙切片图像以生成作为R代码输入的核定位数据（点云）的StarDist机器学习模型。