Removal of SPO11 and Resection of 5' Ends of DNA (yeast)

SPO11 forms a dimer and each subunit cleaves a single strand of DNA, thus creating a double-strand break. After cleaving DNA, a SPO11 subunit remains covalently attached to each 5' end via a tyrosine residue. SPO11 is removed from the DNA by cleavage of single strands 3' to the attached SPO11. The products are a resected 5' end (protruding 3' overhang) and a covalent complex of SPO11 with an oligonucleotide.

SPO11 蛋白形成二聚体，每个亚基切割 DNA 的单链，从而产生双链断裂。切割 DNA 后，SPO11 亚基通过酪氨酸残基与每个 5' 端以共价键结合。SPO11 通过切割连接到其上的 3' 单链从 DNA 上被去除。产物包括被切除的 5' 端（3' 端突出）和 SPO11 与寡核苷酸形成的共价复合物。