The RNA-binding proteins Roquin-1 and Regnase-1 interact and cooperate to prevent autoimmunity

This study aimed to analyze the transcriptomic changes occuring after knockout of alleles encoding for the RNA-binding proteins Roquin-1 and Roquin-2 (DKO), Regnase-1 (KO) or a combination of all three (TKO) in Th1 cells without or after restimulation with anti-CD3/28 antibodies. Data analysis revealed that in unstimulated as well as restimulated T cells of all three knockout genotypes a number of genes is differentially expressed compared to WT cells, with the strongest deregulation seen in TKO cells. A comparison of gene expression changes of all three genotypes indicated that cooperativity of Roquin and Regnase-1 is the prevalent mode of target regulation. Overall design: RNA Seq of WT, DKO, KO or TKO of CD4 T cells without or with restimulation with anti-CD3/28 for 1h, 2h, 4h