BCCZO -- Soil Microbes -- Gordon Gulch -- (2011-2011)

In this study conducted by Kathryn Eilers, distributions of Bacteria and Archaea were conducted within Upper and Lower Gordon Gulch. The purpose of this study is to discover how the distributions are related to slope aspect, vegetation type, and edaphic factors.

Sampling was conducted along five transects approximately 200m (transects 1, 2, 3, 6 and 7), 400m (transects 8, 9, and 10), or 600m (transects 4 and 5) in length in the two regions of the watershed (see Overview Map) with samples collected from twenty, regularly spaced points (10 m, 20 m, or 30 m apart) along each transect. Each sample consisted of twenty, 0-5 cm cores, taken within a one meter radius, that were homogenized and sieved to 2 mm. Samples were then stored at 40C and split for analysis within two days of collection. Samples used for soil microbial community analysis were frozen at -800C until DNA was extracted.

在本项由凯瑟琳·艾利斯所进行的研究中，对上戈登峡谷和下戈登峡谷的细菌与古菌分布进行了调查。研究旨在揭示分布状况与坡向、植被类型以及土壤学因素之间的关联。采样活动沿五个样带进行，样带长度分别为约200米（样带1、2、3、6和7）、400米（样带8、9和10）或600米（样带4和5），在流域的两大区域内进行（参见概览图）。在每个样带上，从等距离排列的二十个点（间距为10米、20米或30米）收集样本。每个样本由二十个0-5厘米的土芯组成，这些土芯是在半径一米的范围内采集的，经过均质化处理后筛选至2毫米。随后，样本被储存在40摄氏度下，并在采集后两天内进行分割分析。用于土壤微生物群落分析的样本则被冷冻至-80摄氏度，直至提取DNA。