Heritability and Reversibility of DNA Methylation Induced by in vitro Grafting between Brassica juncea and B. oleracea

We found that compared with the expression of siRNAs in leaves of TTT, 3284, 4789, and 6208 unique siRNAs were significantly up-regulated, and 3420, 3850, and 4245 unique siRNAs were significantly down-regulated in GS1, GS3, and GS5, respectively through the high-throughput sequencing of small RNAs Overall design: Small RNA sequencing were performed in tuber mustard (TTT) and selfed progenies GS1, GS3, GS5 (GS=grafting-selfing, n=generations) of TTC [LI-LII-LIII, LI=outer layer of shoot apical meristem (SAM), LII=middle layer, LIII=inner layer, T=tuber mustard, C=red cabbage] was created by in vitro grafting between tuber mustard and red cabbage. Two biological replicates per sample combined 3 plants each