We describe an affinity purification–mass spectrometry (AP–MS) method for probing the interactome of a special targeting protein. The AP was implemented with monolithic micro immobilized metal ion aff

Figshare2020-01-29 更新50

Data_Sheet_1_Impaired interactions of ataxin-3 with protein complexes reveals their specific structure and functions in SCA3 Ki150 model.docx

神经退行性分子机制

蛋白质相互作用组学

Spinocerebellar ataxia type 3 (SCA3/MJD) is a neurodegenerative disease caused by CAG expansion in mutant ATXN3 gene. The resulting PolyQ tract in mutant ataxin-3 protein is toxic to neurons and curre

NIAID Data Ecosystem30

Versatile regulation of autophagy by the deubiquitinase USP11

自噬分子调控

蛋白质相互作用组学

Ubiquitination is a versatile post-translational modification known to regulate autophagy. Here, we show that loss of the deubiquitinase USP11 leads to an increase in the autophagic flux. To investiga

NIAID Data Ecosystem60

Additional file 6 of Splicing-accessible coding 3′UTRs control protein stability and interaction networks

蛋白质相互作用组学

质谱分析

Additional file 6: Table S5. Interactions of U2AF26fl and U2AF26fs analyzed by mass spec.

NIAID Data Ecosystem40