Gene expression profile at single cell level of FACS sorted CD8+ T cells from subcutaneous MC38 tumors from WT and Susd2-/- mice. Gene expression profile at single cell level of FACS sorted CD8+ T cells from subcutaneous MC38 tumors from WT and Susd2-/- mice

Tumor-infiltrating CD8+ T cells are phenotypically heterogeneous with varying degrees of functional defects. We used single cell RNA sequencing (scRNA-seq) to analyze their sex differences. Overall design: Tumor-infiltrating CD8+ T cells in WT and Susd2-/- mice were isolated by Fluorescence-Activated Cell Sorting (FACS) from MC38 s.c. tumors on Day 18 post inoculation according to their Cd8a, Trbc1, and Trbc2 expression and analyzed using scRNA-seq