84-B10 alleviates cisplatin-induced acute kidney injury in mice

Mice were pre-treated with different doses of 84-B10 for 48h before cisplatin (25mg/kg, intraperitoneal) challenge in both cisplatin and cisplatin +84-B10 group, then treatment groups were received 84-B10 daily. After 72h cisplatin challenge, mice of all groups were sacrificed and RNA-Seq were performed. Cisplatin was induced as described in the previous section (n = 4 in each group). The mice were pretreated with 84-B10 for 48 h in cisplatin + 84-B10 group at a dose of 15 mg/kg before cisplatin treatment.Cisplatin was challenged to the mice by aintraperitoneal (i.p.) injection (25 mg/kg) in both cisplatin and cisplatin + 84-B10 group. The vehicel mice received an i.p. injection of saline. Kidney tissues were harvested and perform RNA-Seq.