File S1 - Integration of Molecular Profiling and Chemical Imaging to Elucidate Fibroblast-Microenvironment Impact on Cancer Cell Phenotype and Endocrine Resistance in Breast Cancer

Figures S1-S9. Figure S1. Amphiregulin mRNA expression is increased in MCF-7 breast cancer cells in the sandwich co-culture (MCF-7S). Figure S2. Light microscopy image showing fibroblasts recruited to MCF-7 spheroids after 3 and 5 days in culture. Figure S3. Comparison of morphology and response to E2 in 2D and 3D MCF-7 models. (A) Micrograph of MCF-7 cells grown in 2D and 3D culture. (B) Response of MCF-7 cells to E2 stimulation is similar in 2D and 3D cultures as monitored by induction of E2 response genes progesterone receptor (PR) and Ki67. Figure S4. Table of DAVID pathway association based on iSig. Figure S5. Table of ONCOMINE dataset patient characteristics. Figure S6. Expression of iSig is correlated with breast cancer stromal expression. Figure S7. FT-IR signature of E2 response of MCF-7 cells is based on fibroblast density. Figure S8. FT-IR signature of E2 response of MCF-7 cells is affected by treatment with CM. Figure S9. Fibroblasts recruited to the outside of MCF-7 spheroids display mild α-SMA staining. (PPTX)