Expression data from lymphocytes (CD3+ cells) from severe, mild allergic patients and a non allergic group.

The causes underlyed the acquisition and maintainance of a severe allergic phenotype remain unknown. Here, we study the cd3+ complete transcriptomic fingerprint from severity-stratified patients to understand the involvement of these cells in the development of severe allergy. We used microarrays to detail the global programme of gene expression of CD3+ cells from severity-stratified allergic patients We recruited respiratory allergic patients and stratified them acording to severity. A non allergic group was also recruited. Blood samples were obtained by plateletpheresis. PBMCs were isolated by a ficoll graadient. CD3+ cells were isolated by magnetic bead separation. RNA was extracted from CD3+ cells and used for transcriptomics by affymetrix.