Transcriptional profiling of mouse embryonic tissues with Firre deletion

Firre is a long non-coding RNA expressed dynamically in a variety of tissues throughout mouse development. While it is known to play a role in chromatin organization, its physiological relevance remains unclear. In order to better understand the role of Firre in vivo, we generated Firre knockout mice and performed RNA sequencing and differential expression analysis on eight tissues from both males and females at E11.5. RNA sequencing in triplicate of eight E11.5 tissues from male and female mice with Firre knockout.Firre是一种在小鼠整个发育阶段中于多种组织内动态表达的长链非编码RNA（long non-coding RNA）。尽管已知其在染色质组织过程中发挥作用，但其生理相关性仍未明确。为了更深入地解析Firre在体内的生物学功能，我们构建了Firre基因敲除小鼠，并对胚胎发育第11.5天（E11.5）的雌雄小鼠的8种组织开展了RNA测序（RNA sequencing）与差异表达分析。对Firre基因敲除雌雄小鼠的8种胚胎发育第11.5天组织进行了三次重复的RNA测序。