Mouse BM ILC progenitor subsets

Scrna-Seq was performed on BM Lin- CD127+ cells Overall design: Parenchyma and Sinusoid bone marrow Lin- CD127+ cells were stained with Mouse Immune Single-Cell Multiplexing Kit and loaded onto a BD Rhapsody cartridge. cDNA synthesis was performed using the BD Rhapsody Express Single-Cell Analysis System, and gene expression libraries were prepared using Whole Transcriptome Analysis Amplification Kit (all from BD Biosciences).