Additional file 4: of An internet-based bioinformatics toolkit for plant biosecurity diagnosis and surveillance of viruses and viroids

Paired sample t-test of contigs produced by SPAdes, Velvet, ABySS and SOAPdenovo assemblers. Our optimised ‘SPAdes K15-17-19_cap3’ (SPAdes assembly; dataset = 21–22 nt; kmer set =15,17 and 19; and scaffolded with CAP3) (SP22_K15-17-19_cap3) assembly was compared against all other kmer settings and/or tools. A) SPAdes - Longest assembled contigs (21–22 nt pipeline); B) SPAdes - Total number of assembled bases for contigs > =100 nt (21–22 nt pipeline); C) SPAdes - Longest assembled contigs (21–25 nt pipeline); D) SPAdes - Total number of assembled bases for contigs > =100 nt (21–25 nt pipeline); E) Comparison of longest assembled contig using Velvet against SP22_K15-17-19_cap3; F) Comparison of longest assembled contig using ABySS against SP22_K15-17-19_cap3; G) Comparison of longest assembled contig using SOAPdenovo against SP22_K15-17-19_cap3. (XLSX 19 kb)