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Detection of IgG anti-Leishmania antigen by flow cytometry as a diagnostic test for
cutaneous leishmaniasis
Figure legend
Figure 1. Gate strategy to determine beads population
to be analyzed. A gate was performed
based on FSC and SSC and than mean florescence intensity was assessed based on
FITC staining.
Figure 2. Antibody titers assessed by ELISA. Anti-SLA IgG titers from healthy subjects (HS),
cutaneous leishmaniasis patients (CL), Montenegro positive individuals (DTH+)
and Chagas disease patients (CD), assessed by ELISA. Cut off was determined by
a ROC curve containing absorbances from CL patients and healthy subjects. OD,
optical density.
Figure 3. Antibody titers assessed by flow cytometry. Anti-SLA IgG titers from healthy subjects (HS),
cutaneous leishmaniasis patients (CL), Montenegro positive individuals (DTH+)
and Chagas disease patients (CD), assessed by flow cytometry. Cut off was
determined by a ROC curve containing MFI from CL patients and healthy subjects.
MFI, mean fluorescence intensity.
创建时间:
2016-08-31



