The transcriptome analysis of WUS targets

WUS is a key transcription factor and plays important role in plant stem cell maintenance. WUS expresses in organizing center and its protein moves upward to stem cells to activate CLV3 expressions. Only a few target genes of WUS were reported which involved in stem cell maintenance. To indentify new targets of WUS, we performed RNA high throughput sequencing after inducing WUS activity with DEX (dexamethasone) and CHX (Cycloheximide). The 4-week-old PUBI10:Mcherry-GR-Linker-WUS plants were inoculated with Cucumber mosaic virus (CMV) in leaves, the shoot apexes were collected at 8dpi. The PUBI10:mCherry-GR-Linker-WUS plants without CMV inoculation were defined as the mock treated. The shoot apexes with or without CMV infection were treated with CHX (10 μM) alone or CHX+ DEX (15μM) for 2 hours, and then total RNA were isolated from the shoot apexes. Strand-specific RNA-seq libraries were prepared and sequenced on the Illumina Hi-Seq2500 platform. Reads were aligned to the Arabidopsis TAIR10 reference genome, and differentially expressed genes were identified using DEG seq. Two biological replicates were performed for RNA-seq.