Raw sequencing, breseq analysis, and PBP 1A/1B protein sequence alignments. Used in the article "Deep-learning-based deconvolution and segmentation of fluorescent membranes for high precision bacterial cell size profiling"

The repository includes the following data from the publication "Deep-learning-based deconvolution and segmentation of fluorescent membranes for high precision bacterial cell size profiling". 1) Raw sequencing of Priestia megaterium WH320. Illumina pair-end sequences were loaded into Geneious prime version 2025.1.2 for sequence trimming and pairing with an insert size of 500 base pairs. Paired sequence list was then exported in fastq format which is the file deposited here. 2) breseq analysis of WH320 mapped to DSM 319's reference genome. breseq version 0.35.5 was used. 3) ponA protein sequence alignments (WH320, DSM 319, and >800 downloaded from UniProt). The UniProt query was "ponA" and restricted to bacteria taxa only, using a sequence length threshold between 700 and 1000 aminoacids. Sequences were loaded into Geneious prime version 2025.1.2 and aligned using Clustal Omega 1.2.3, then exported as a .fasta file which is the file deposited here.