Relative expression of mRNA transcripts related to hippocampal plasticity after repeated exposure to extremely-low frequency electromagnetic field (50 Hz)

Methodology:PCR reactions were performed with LightCycler®96 apparatus by Roche.Data was acquired from the apparatus and processed with LightCycler® software (Roche, ver. 1.1)The efficiency of each reaction was calculated with LightCycler® software (Roche, ver. 1.1) with series of dilution of cDNA matrix mixThe quality of the procedures was ensured by usage of triplicates of each sample.Glyceraldehyde 3-phosphate dehydrogenase was used as a housekeeping gene.Data-specific information:Number of variables: 2Variable list:1. Electromagnetic field intensity - 1) 1 mT; 2) 7 mT;2. Number of exposures - 1) one; 2) two; 3) three;Abbreviations:E1 - single (seven consecutive days, 1h/day) exposureE2 - double (2 times of seven consecutive days, 1h/day, with three weeks break in between) exposureE3 - triple (3 times of seven consecutive days, 1h/day, with three weeks break in between) exposureC - control groupELF-EMF/1 mT - group exposed to an extremely-low frequency electromagnetic field of 1 mTELF-EMF/7 mT - group exposed to an extremely-low frequency electromagnetic field of 7 mTCt - cycle thresholdBak1 - Bcl-2 homologous antagonist killerBcl-2 - B-cell lymphoma 2Bcl-xL - B-cell lymphoma-extra largeBDNF - brain-derived neurotrophic factorGAP43 - growth associated protein 43PSD95 - postsynaptic density protein 95SYN1 – synapsin ITrkB - tropomyosin receptor kinase B