Analysis of chromatin occupancy in erythroid cells by CUT&Tag following CRISPR editing of BCL11A enhancer

The mechanisms underlying the remarkable efficacy of CRISPR-mediated enhancer ablation remain poorly understood. Here, we employ CUT&Tag to investigate histone modifications following BCL11A enhancer editing or CTCF disruption. Overall design: CUT&Tag analysis of H3K27ac was performed in wild-type HUDEP-2 cells, enhancer knockout cells, sg1617-edited cells, and double CBS knockout single-cell clones, each with two independent biological replicates. CUT&Tag analysis of NIPBL was perfromed in wild-type HUDEP-2 cells, sg1617-edited cells, and ASO treated cells. Additionally, CUT&Tag of H3K27ac was conducted in control AAVS-edited and sg1617-edited CD34+ HSPCs following erythroid differentiation.