Apparent association and dissociation kinetic data for the binding of PVP1–63-EGFP to immobilized S. Typhimurium LT2 cells.

Two different concentrations (5 and 10 µM) were measured in three-fold and the corresponding average is represented. The association rate (ka1), dissociation rate (kd1), forward rate (ka2) and backward rate (kd2) constants are calculated according to a two-state model that describes a 1∶1 binding (AB) of the fusion protein analyte (A) to the immobilized ligand (B), followed by a conformational change in the complex (AB→AB..).