Quantitative proteomics analysis of paired colorectal cancer and non-tumorigenic tissues reveal proteins and pathways perturbed in colorectal cancer

Proteomics is a dynamic field emerged dramatically the post genomic era which has been extensively employed to study altered protein expression to gain insight into the development process and pathways involved in cancer and uncover potential protein markers. Membrane proteomes of an expanded cohort of paired CRC and adjacent non-tumorigenic tissues (1 female and 7 males) were profiled using high resolution nanoLC-MS/MS-based proteomics, which identified 1,000-1,300 proteins in each of the two tissue types. 184 proteins were differentially expressed (P < 0.05, fold change > 1.5); 69 proteins were up- regulated and 115 proteins were down-regulated in the CRC tissues relative to non-tumorigenic tissues.