Genome sequencing of Ustilago maydis ITA-Chassis strain. Mycosarcoma maydis

Ustilago maydis wildtype strains generally produce a potpourri of metabolites, which hinders efficient production of single target chemicals. In this study, the diverse by-product spectrum of U. maydis was reduced through strain engineering using CRISPR/Cas9 and FLP/FRT, greatly increasing the metabolic flux into the targeted itaconate biosynthesis pathway. Whole-genome sequencng was performed to get a deeper understanding into phenomena originating from the cloning techniques used.