single-cell sequencing of holo-transcriptome

We introduce a single-cell holo-transcriptome sequencing (Holo-Seq) that has the same quantitative accuracy and uniform coverage with a complete strand of origin information as bulk RNA-seq. Most importantly, Holo-Seq can simultaneously observe small-RNAs and mRNAs in a single cell. Furthermore, we acquire small-RNA and mRNA dual transcriptomes of 32 human hepatocellular carcinoma single cells, which display the genome-wide super-enhancer activity and hepatic neoplasm kinetics of these cells.