Gene expression data from CD8+ T cell subsets

As humans age, their memory T cell compartment expands due to the lifelong exposure to antigens. This expansion is characterized by the presence of terminally differentiated CD8+ T cells (Temra), which possess NK cell-like phenotype and are associated with chronic inflammatory conditions. Temra cells are predominantly driven by the sporadic reactivation of cytomegalovirus (CMV), yet their epigenomic patterns and cellular heterogeneity remain understudied. To address this gap, we correlated their gene expression profiles with chromatin openness and conducted single-cell transcriptome analysis, comparing them to other CD8+ subsets and CMV-responses. PBMCs were stimulated before cell sorting with CMV pp65 peptide pool (PepTivator) for 16-24h in CO2 enriched thermostat at 37C. CD8+ T cell subsets from 28 different individuals (>65 y old) were sorted with MA900 Multi-Application Cell Sorter (Sony Biotechnology) and collected at 4C into collections tubes containing TRIzol reagent. 96 samples were subjected to microarray analysis of which 91 had a good quality. We analysed altogether 4 CD8+ T cell subsets of which 12 were CMV-reactive T cells, 24 naive T cells, 27 memory T cells and 28 Temra cells.