MYC ChIP-seq in human pancreatic ductal adenocarcinoma cells

To assess modulation of global MYC chromatin occupancy by stromal cues, we exposed human pancreatic cancer cells to secreted factors from cancer-associated fibroblasts and performed ChIP-seq in stroma-stimulated cells compared to controls. Overall design: MIAPaCa2 human PDAC cells were serum-starved for 48 hours, then treated with fresh serum-free media or with serum-free conditioned media from primary cancer-associated fibroblasts. After 3 hours, cells were fixed and ChIP for MYC performed.