Gene Expression Analysis of Host Immune Responses in rhesus macaques infected with SIVmac239 or deglycosylated live-attenuated SIV

To find the differences in innate immune response between nonpathogenic and pathogenic SIV infection, we examined transcriptome in PBMCs from macaques infected with pathogenic or nonpathogenic SIV. Juvenile macaques were intavenousy infected with SIVmac239 or ∆5G. Blood samples were collected at 1 week and 3 weeks pi. Total RNA from the PBMCs were used for the microarray analysis. cRNA was hybridized to a rhesus microarray (Agilent ver.2). The data were normalized per chip to the 75 th percentile expression level, then subjected to per gene to normalization to the median expression of uninfected animals.