Relative amounts of various modified nucleosides of total tRNA isolated from the trm9Δ strain before and after methylation reaction.
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Pseudouridine (Ψ) was used as the internal control. The numbers displayed are the ratios (modified nucleoside/Ψ). ND: not detected. The modified nucleosides cm5U, ncm5U, mcm5U and Ψ were monitored at 254 nm, and cm5s2U, ncm5s2U and mcm5s2U were monitored at 314 nm as thiolated nucleosides absorb well at this wavelength, while nonthiolated nucleosides do not.
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2015-12-02



