Single-cell RNA-Sequencing data of posteriorized embryonic-like sacs, amniotic ectoderm-like cells, and H9 hESC

The study of early post-implantation human embryo development is limited by the scarcity of embryo samples and ethical restrictions. We developed a human pluripotent stem cell (hPSC)-based microfluidic platform to model several developmental processes during this stage. We applied single-cell mRNA sequencing technique to examine the transcriptomes of posteriorized embryonic-like sacs obtained using the microfluidic platform, as well as of amniotic ectoderm-like cells generated using a Transwell method and human embryonic stem cells (H9 hESCs). Overall design: Cells retrieved from posteriorized embryonic-like sacs were pooled from 6 microfluidic devices, after 48 hours of exogenous BMP4 stimulation. Amniotic ectoderm-like cells were also obtained by treating H9 hESCs with BMP4 for 48 hours using a Transwell method. H9 hESCs maintained using standard tissue culture dishes were also sequenced for comparison.