Adaptive and innate immune responses in MAC lung disease

Immunological mechanisms of susceptibility to NTM disease are poorly understood. We evaluated innate and antigen-specific adaptive immune responses to Mycobacterium avium complex in individuals with MAC lung disease. We investigated the gene expression changes induced in PBMCs by polyclonal stimulation (anti-CD3, anti-CD28), Mav and Mtb lysates and a peptide pool (MTB300) in individuals with MAC lung disease (MACDZ), and healthy controls (IGRA+ and IGRA-). Overall design: RNAseq of sorted PBMC from cryopreserved PBMC of IGRA+ Healthy controls, IGRA- Healthy controls and individuals with MAC infection (MACDZ). Mtb300 has been published in this paper: https://pubmed.ncbi.nlm.nih.gov/27409590/