Formation of hematopoietic colonies by CD34+ cells that were derived from EMS-iPS cells or control iPS cells co-cultured with AGM-S3 cells.

EMS-iPS cells and control iPS cells (control 1: 201B7, control 2: TkDA3-1) were co-cultured with AGM-S3 cells. After 12 days, cells derived from iPS cells were collected. CD34+ cells were isolated for clonal hematopoietic culture. After 12 days, colonies were examined. The mean numbers of colonies ± SD of triplicate cultures are showed. G, granulocyte; M, macrophage; GM, granulocyte-macrophage; E, erythroid; and Mix, mixed-lineage colonies.*p + cells derived from control iPS cells (Student’s t-test).Formation of hematopoietic colonies by CD34+ cells that were derived from EMS-iPS cells or control iPS cells co-cultured with AGM-S3 cells.