Analysis of microbiomes and drug-resistance mutations detected by HC-based NGS among HIV-infected patients with pneumonia

This study utilized hybrid capture-based next-generation sequencing (HC-based NGS) to analyze lung microbiomes and Drug Resistance Mutations of HIV and bacteria in people living with HIV (PLWH) with pneumonia, explore the microbiome spectrum of AIDS combined with pulmonary infection in central China, and the applicability of HC-based NGS for genotyping resistance testing of HIV and bacteria. We retrospectively investigated lung microbiomes in PLWH hospitalized at Zhongnan Hospital of Wuhan University from 1 may 2022 to 30 December 2022. A combination of Bronchoalveolar Lavage Fluid (BALF) HC-based NGS and conventional microbiological tests (CMTs) were performed in all patients.