Evaluation of four DNA extraction kits for implementation of Nanopore sequencing

This study aimed to optimize DNA extraction protocols for ONT sequencing and to assess the practical feasibility of implementing MinION sequencing as a tool for AMR surveillance in resource-limited settings. We evaluated four commercial DNA extraction kits using both Gram-positive and Gram-negative bacterial isolates, covering different lysis (enzymatic, chemical, and bead-beating) and purification strategies (silica spin-column, magnetic bead-based, and glass bead-based).