qPCR data analysis.pzf

Raw qPCR data and data analysis fileExpression
profile of (a) hsa-miR-143-3p, (b) its predicted targets, (c) stem cell
markers, (d) differentiation marker and (e) Wnt signaling regulators upon
transfection with hsa-miR-143-3p mimic and inhibitor. Relative mRNA / miRNA
expression in mimic transfected and inhibitor transfected cells were quantified
in comparison to control by qPCR using SYBR Green chemistry. Each sample (n =
3) was run in triplicate. The data were expressed as mean ± SD and relative
fold change of expression (RQ) was calculated by 2-∆∆CT method after
normalization with GAPDH (reference gene)/ RNU6B (reference miRNA). p < 0.05 was considered to be statistically
significant.