NEFA exposure of bovine oocytes significantly reduced the oocyte developmental competence and the quality of the resultant blastocysts.

Oocytes (n = 1024; three replicates) were matured in maturation medium supplemented with 1) physiological NEFA  =  control (150 µM of total NEFA, i.e. OA, SA and PA); 2) elevated Stearic Acid  =  HIGH SA (75 µM SA); 3) elevated Palmitic Acid  =  HIGH PA (150 µM PA) and 4) elevated Oleic Acid  =  HIGH OA (200 µM OA). Data marked with different superscripts per row are significantly different between treatments (P<0.05).