Nor climate, nor human impact factors: Chytrid infection shapes the skin microbiome of an endemic amphibian along a biodiversity hotspot

The amphibian skin microbiome is a key component of the host’s innate immune system. However, we lack a complete understanding of the mechanisms by which different drivers can alter this function by modulating the microbiome’s structure. Our aim was to assess the extent to which different host attributes and extrinsic factors influence the structure of the skin microbiome. Skin bacterial diversity was examined in 148 individuals of the four-eyed frog (Pleurodema thaul) from 16 localities spanning almost 1,800 km in latitude. Alpha (richness) and beta diversity and abundance of bacterial amplicon sequence variants were used to describe its structure. Predictors associated with the host (developmental stage, genetic lineage, individual Batrachochytrium dendrobatidis [Bd] infection status) and the landscape (current climate, degree of anthropogenic disturbance) were used in the statistical modelling in an information theoretical approach. Bd infection and host developmental stage were the ..., , , # Nor climate, nor human impact factors: chytrid infection shapes the skin microbiome of an endemic amphibian along the Chilean biodiversity hotspot

## Description of the Data and file structure

The dataset consists of 3 CSV files with the corresponding R-scripts.

### Alpha diversity

The dataset \"Alpha_diversity.csv\" contains 148 individual bacteriome richness data for 16 different localities as ell as other relevant information associated with each of the 148 sampled individuals.

#### Column names

SR: bacteriome family richness (cuantitative: number of families)
BD: whether the individual was infected or not with Bd (categorical: yes/no)
HIF: Human footprint for a locality (cuantitative: no units)
Year: year when the sample was collected (cuantitative)
LOCATION: locality of sampling (categorical)
Lineage: host genetic lineage of the sampled individual (categorical)
bio1: annual mean temperature (cuantitative: ºC)
bio4: temperature seasonality (cuantitative: ºC/100)
bio12: annual ...