Transcriptomic analysis of the Xrn1 depletion by Auxin-degron shutoff
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE123326
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The goal of the project was to study the effects on transcription and mRNA stability of the Xrn1 sudden depletion. We analyzed the effect of Xrn1 depletion caused by protein degradation of an Auxin-degron fusion on the transcription rates, mRNA stabilities and mRNA levels by doing Genomic Run-On (GRO) experiments at 30 min after Auxin addition with a control at 0 min. Cells were grown in YPD at 30ºC to 0.5 OD600. At 0 min and 30 min after Auxin addition cells were spun-down and collected. Please note that each sample record represents three replicates (more details in the sample description field).
创建时间:
2019-04-02



