Single-cell analysis of the epigenomic and transcriptional landscape of innate immunity to seasonal and adjuvanted pandemic influenza vaccination in humans (TIV_scATAC-seq)

Human subjects were vaccinated with seasonal influenza vaccine (TIV, 2014-2015). We then used scATAC-seq to construct the single-cell landscape of the innate immune response to TIV at the epigenomic level. PBMCs from vaccinated individuals were isolated at day 0, 1, and 30, then enriched for DC subsets using flow cytometry and analyzed using droplet-based single-cell chromatin accessibility profiling. A total of 9 samples from 3 healthy individuals at 3 time points was analyzed: day 0 before vaccination, and day 1 and day 30 after vaccination.