Sex-specific vascular effects of menthol: TRPM8 and TRPA1-dependent relaxation in female mouse aorta and pudendal arteries

Sexual dysfunction affects the quality of life for both men and women. Menthol, a TRPM8 agonist, is widely used in products to enhance sexual performance due to its cooling effect. Beyond this, menthol also induces vascular effects in male arteries. To explore its effects on female vasculature, we studied male and female C57BL/6 mice, along with female TRPM8 knockout (KO) mice. We isolated the internal pudendal artery and aorta to assess isometric contractile force. Menthol-induced relaxation of the pudendal artery was reduced in both female TRPM8 KO and male mice compared to female controls. Acetylcholine-induced relaxation was not affected in females, but in males, it was attenuated by menthol and enhanced by M8-B, a TRPM8 inhibitor. Menthol did not alter responses to norepinephrine, serotonin, or histamine in either sex. Notably, menthol-induced relaxation was also reduced in the aortas of female TRPM8 KO mice. In both the aorta and pudendal arteries of femal..., , # Data from: Sex-specific vascular effects of menthol: TRPM8 and TRPA1-dependent relaxation in female mouse aorta and pudendal arteries

Dataset DOI: [10.5061/dryad.t1g1jwtgv](https://doi.org/10.5061/dryad.t1g1jwtgv)

## Description of the data and file structure

Pudendal and aortic artery rings (2 mm) were mounted on wire or pin myographs and maintained in oxygenated Krebs–bicarbonate solution at 37 °C; pudendal arteries were normalized using dedicated software, whereas aortic rings were set to a passive tension of 5 mN. For vascular reactivity studies, arterial rings were precontracted with phenylephrine, except during norepinephrine and serotonin concentration–response experiments, and cumulative concentration–response curves were constructed for norepinephrine, serotonin, acetylcholine, histamine, or menthol in the absence or following incubation with menthol, M8-B, or HC030031.

All data are presented as mean ± standard error of the mean (SEM), where *n* denotes the number of inde...,