Orbicella faveolata coral metagenome assemblies from the ECA region of Florida, USA

The enclosed files include Orbicella faveolata coral metagenome assemblies collected from the Coral Ecosystem Conservation Area (ECA) in southeast Florida, USA. Metadata for the files including region of collection and associated NCBI accession numbers is included in this repository as the metadata file. Apparently healthy coral tissue cores were collected between May 28 and June 21, 2021. The DNA was extracted from the host tissue and mucus and sequenced in a paired-end 150 bp format on an Illumina NovaSeq. Trimming and quality filtering of DNA sequences proceeded, followed by host and endosymbiotic dinoflagellate DNA removal. The host-cleaned reads were assembled individually by coral sample into longer contigs using MegaHit v1.1.4. The “Assembly_Fastas” zipped file contains 45 metagenome assemblies from the individual Orbicella faveolata corals. In addition, these metagenome assemblies were annotated with eggnog-mapper v2.1.6 to generate both predicted gene regions and annotation output files. The “Predicted_Gene_Fastas” zipped file contains nucleotide fasta files of the predicted gene regions for the 45 coral metagenome assemblies. The fasta header of each gene includes the contig ID it originated from in the associated “Assembly_Fastas”. The “Predicted_Gene_Annotations” zipped file contains either .csv or .xlsx files with the eggnog-mapper-based annotations. These files contain a “query contig” that corresponds to the contig ID in the fasta header of the “Predicted_Gene_Fastas”.  

These data were processed and generated by Julie Meyer’s Lab at the University of Florida, using funding from the Florida Department of Environmental Protection.