Megalobrama amblycephala Raw sequence reads

One control group and three Hypoxic-treatment groups were prepared to sequence, with three replicates in each group. Total RNA was extracted from each tissue using TRIZOL (Invitrogen, Carlsbad, CA, USA) according to the manufacturer's instructions. RNA samples were digested by DNase I to eliminate potential genomic DNA. Integrity and size distribution were checked on a Bioanalyzer 2100 with RNA 6000 Nano Labchips (Agilent technologies, Santa Clara, CA, USA). All the samples were standardized to 500 ng/µl. At each hypoxia and reoxygenation stage, three ploy(A)-enrichment bioreplicated libraries and one miRNA library were constructed. Totally twelve mRNA sequencing libraries and four miRNA sequencing libraries were constructed and sequenced on an Illumina HiSeq 2500 (Illumina, San Diego, USA) with 150-bp paired-end reads(mRNA) and 50 bp single-end(miRNA).