Products of insulin B chain hydrolysis by NEP.
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NEP mediated hydrolysis was carried out as described in Table 2. The reaction was stopped by adding 10 µL of 5% TFA when approximately half of the substrate had been hydrolyzed (180 min.). The acidified reaction mixture was subjected to HPLC as described in figure 1, individual peaks were collected and identified by mass spectral analysis.
创建时间:
2015-12-02



