Mutation detection and estimation of mutation frequency in three candidate genes of Ppd-D1, Rubisco activase A and Rubisco activase B by TILLING analysis.

a, mutation detected by non-denaturing polyacrylamide gels stained with silver;b, mutation detected by non-denaturing polyacrylamide gels stained with ethidium bromide;c, mutation detected by agarose gels stained with ethidium bromide.*For calculation of the mutation frequency, 100 bp sequences from each end were removed due to the base ambiguity.