Isolation of infectious MAYV from RM plasma and tissue.
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Using NHP tissue homogenates collected in PBS at 10 dpi, we infected C6/36 cells and harvested supernatants at 3 dpi to isolate infectious MAYV. Viral supernatants were tittered in triplicate by limiting dilution plaque assays on Vero cells to quantify infectious viral particles in cell supernatants. Viral titers are reported as plaque forming units per 1 mL of C6/36 viral supernatant. The LOD was 3.3 PFU/mL. Samples with titers below the LOD are labeled (-).
创建时间：
2023-11-20
