Comparative transcriptomic response analysis of Escherichia coli K-12 MG1655 to nine antibiotics

To investigate and compare transcriptomic changes of Escherichia coli K-12 MG1655, the bacterium was exposed to nine antibiotics (tetracycline, mitomycin C ,imipenem, ceftazidime, kanamycin, ciprofloxacin, polymyxin E, erythromycin, and chloramphenicol) , and RNA-Seq was performed to determine comparative transcriptomic changes. Escherichia coli K-12 MG1655 was grown in MHB medium supplemented with antibiotics at concentrations that inhibit 50% growth (tetracycline 1.8 mg/L, mitomycin C 1.4 mg/L, imipenum 0.06 mg/L, ceftazidime 0.12 mg/L, kanamycin 1.6 mg/L, ciprofloxacin 0.012 mg/L, polymyxin E 0.4 mg/L, chloramphenicol 1.1 mg/L, erythromycin 7 mg/L). Cells were grown to mid-log phase, havested, and subject to RNA-Seq analysis. Controls were performed (ethanol for erythromycin and chrloamphenicol as they are dissolved in ethanol, and water for every other antibiotic). Triplicates were performed.