miRNA expression profifile during osteogenic differentiation of hPDLSCs was analyzed using microarray

The roles of miRNA in osteogenic differentiation of human periodontal ligament stem cells (hPDLSCs) remain largely unexplored. In this study, the underlying molecular mechanism of osteogenic differentiation in hPDLSCs is investigated using microarray profiling. miRNA expression of hPDLSCs at differentiated group were cultured with an odontogenic differentiation medium containing 100 nM dexamethasone, 10 mmol/L β-glycerophosphate, and 50 mg/mL ascorbic acid. (Sigma, St Louis, MO, USA) for 14 days in 6-well plates. hPDLSCs at undifferentiated group were cultured in 10 % FBS in DMEM with no supplements.